Poster Presentations
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Gold nanoparticle chemiresistor sensor for providing antibacterial susceptibility profile
Mohammadkazem Papan, Phd Student, Macquarie University/ CSIRO
Diagnostics have critical role in managing the challenge of antimicrobial resistance.
Most of the commercially rapid methods for identifying antimicrobial resistance are genotypic methods. Genotypic methods provide information for predicting antimicrobial resistance not antimicrobial susceptibility. Chemiresistor sensors are amongst new technologies being investigated to meet present needs for diagnosing diseases. Potential advantages of the technology include its amenability to point of care diagnostics, inexpensive components and rapid response times we present work on utilizing gold nanoparticle chemiresistors for providing antibiotic susceptibility profile. The detection principle is based on the distinct metabolic differences associated with species specific bacterial growth. Four different bacteria including gram positive and gram negative and more importantly different strains of same pathogen which have different antibiotic susceptibility pattern has been tested with the sensor array. Also, as proof of concept, the sensor is capable enough to provide Minimum Inhibitory Concentration (MIC) results for different pathogens. Limit of detection for different inoculum size has also been measured. To the best of our knowledge this is the first documentation of using AuNP chemiresistors for determining antibiotic susceptibility profile. The results suggest that detection may be possible in less than 24 h which would be a significant reduction in time compared to the 2 to 5 days it can take to identify infections using conventional culture methods.
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