Biomarker Co-localization Measurements (C-NTA) Using the New Particle Metrix ZetaView x30 Family
Sven Rudolf Kreutel,
Sales Director,
Particle Metrix GmbH
During the last decades, Nanoparticle Tracking Analysis (NTA) has
emerged as a vital and fast characterization technology for biological
nanoparticles like Extracellular Vesicles, Exosomes and Viruses. While
classic NTA scatter operation feeds back particle size and total
concentration, the fluorescence detection capability enables the user to
gain specific biochemical information. Determination of biomarker
co-localization however, is a challenging task for any analytical
instrument. A new laser generation ensuring perfect overlap of the
illumination volumes of individual channels paired with very fast
switching times between fluorescence channels lay the foundation of
co-localization nanoparticle tracking analysis (C-NTA) introduced
recently by Particle Metrix. For the first time, we report results of
co-localization measurements of reference material as well as real
biological samples based on NTA technology on the new ZetaView® PMX-230
TWIN.
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