Applications for Small Particle Analysis Using High Sensitivity Flow and Imaging Flow Cytometry
Haley Pugsley,
Manager and Senior Scientist,
Luminex Corporation
Research in the fields of virology, microbiology, nanoparticles, and
extracellular vesicles (EVs) has shown tremendous growth over the past
few years. Viruses, which range in size from 17 nm to ~1.5 microns,
small bacteria, nanoparticles, and small EVs such as exosomes (less than
150 nm in diameter), are all on the subcellular scale. Quantifying and
characterizing small particles in a reproducible and reliable manner is
challenging due to their small size. In this presentation, we will
demonstrate the capabilities for small particle applications from Amnis®
CellStream® and Amnis® ImageStream®X
Mk II. The Amnis CellStream system has the advantage of high throughput
flow cytometry with higher sensitivity to small particles due to the
CCD-based, time-delay-integration image capturing system. Here, we will
present immunophenotyping data from EVs. The Amnis ImageStreamX Mk II
that combines the quantitative power of flow cytometry with microscopy
in one system has a High Gain mode to increase the sensitivity for small
particles by adjusting the CCD-camera to a higher gain setting,
increasing the signal obtained from the small particles while minimizing
the noise. In this portion of the presentation, examples of High Gain
mode using murine leukemia virus-sfGFP will be shown.
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