Poster Presentations
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METABOLOMIC APPROACH OF CARDIOVASCULAR RISK IN CHRONIC KIDNEY DISEASE PATIENTS
Maria Posada Ayala, , IIS-FJD
The identification of novel biomarkers of cardiovascular disease (CVD) is approached to facilitate early diagnosis and prevent future cardiovascular events. Metabolomic strategies are investigated to provide with a global analysis of the whole set of metabolites in the biological sample, without pre-selection of potential targets. The strategy focus to diagnosis in urine, as biological fluid rich in potential biomarkers which does not require invasive intervention. Chronic kidney disease (CKD) patients are selected as high-risk population, distinguishing between those who had or had not suffered a previously reported CV event and compared to healthy individuals.
First morning urine from 15 CKD patients (6 of which had suffered CV event) and from 15 healthy subjects was collected. Differential analysis of urine metabolites was performed by nuclear magnetic resonance (NMR). The data was processed by R and Amix Bruker statistical software, resulting in 25 chemical shifts significantly different. 2D-NMR experiments were carried out over samples pool and 32 metabolites were identified. Urine samples from a new cohort of individuals were analyzed by Chip-LC-MS in SRM mode for validation, confirming involvement of at least 15 metabolites in kidney disease and 5 related to cardiovascular disease.
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