Poster Presentations
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Brain Organoid Perfusion Platform
Graham ROBERTSON, Research Associate, University of Luxembourg
Brain organoids have emerged as an interesting technique for studying brain development and neurological disorders as they more closely mimic the cells’ natural environment in a way that is not possible using standard 2D techniques. However, organoids often require time consuming and delicate maintenance over several weeks or months before they are mature enough for testing. Here we propose a new miniaturised bioreactor that uses perfusion to maintain these delicate structures.
An array of miniaturised bioreactors were designed, arranged into a single device, and fabricated using a From2 SLA 3D Printer. This was used to evaluate the effects of perfusion on organoid viability under perfused, static and control conditions. Premade organoids (DIV30, embedded in Geltrex) were placed into the bioreactors, and either perfused or left in static conditions. Our results show that perfused organoids maintain the same viability compared to standard control conditions in our system. Analysis is ongoing to determine if there are other physiological effects.
We have demonstrated that perfusion of organoids is a practical technique for maintaining their viability. These miniaturised bioreactors are potentially a valuable method for simplified maintenance of organoid cultures, which could enable higher throughput for applications such as drug screening.
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