Molecular Identification of Biosurfactant Producing Bacillus sp Isolated from Sirri Island Petroleum Contaminated Soils
Mohammad Ghayyomi, M.Sc, Cinnagen
Introduction: Biosurfactants are active surface components , that reduce surface and interfacial tension in aqueous solutions and hydrocarbon mixtures.
Materials and Method: Samples of this research were collected from 8 selected points of oil contaminated soils in Sirri Island-IRAN. Primary screening tests including Hemolytic activity, Drop collapse technique, Oil Spreading method were preformed and species with the most positive results were leaded to complementary screening tests like emulsification activity, foaming and surface tension measurement. Selected species were identified by PCR and 16s rRNA Gene Sequence Analysis.
Results:.During primary and complementary screening tests, 29 species showed hemolytic activity, 36 had drop collapsing ability and 19 species showed positive results in emulsification, foaming and surface tension reduction. Finally, four Bacillus species found to be able to reduce surface tension more than 30 mN/m. Then, molecular identification of 11 selected species was preformed based on 16s rRNA gene sequencing, all of them were bacillus sp.
Conclusion:. According to the high potential of Bacillus sp. especially for Microbial Enhanced Oil Recovery (MEOR) and Bioremediation of oil contamination we can hope that further study of the isolates characteristics and looking for new local strains can play an important role in their application in oil industry.
Hot Start dNTPs - Pushing the Limits of PCR
Natasha Paul, Senior Scientific Investigator, TriLink BioTechnologies
Hot Start dNTPs are a unique solution to Hot Start activation in PCR. The Hot Start dNTP technology employs a thermolabile protecting group which blocks low temperature primer extension and is released at higher temperatures to allow for more specific DNA polymerase incorporation. Hot Start dNTPs can be used with a variety of thermostable DNA polymerases and in a number of different reaction formats, including real-time PCR, fast PCR, and multiplex PCR. This presentation will provide an update on the latest advancements in this technology, including the benefits in nucleic acid detection assays.