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SELECTBIO Conferences Organ-on-a-Chip, Tissue-on-a-Chip & Organoids Europe 2020

Organ-on-a-Chip, Tissue-on-a-Chip & Organoids Europe 2020 Agenda

Co-Located Conference Agendas

Lab-on-a-Chip and Microfluidics Europe 2020 | Organ-on-a-Chip, Tissue-on-a-Chip & Organoids Europe 2020 | Point-of-Care, Biosensors & Mobile Diagnostics Europe 2020 | 

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Wednesday, 9 September 2020


Conference Registration, Materials Pick-Up, Morning Coffee and Tea

Session Title: Conference Opening Plenary Session

Plenary Session Venue: Jurriaanse Zaal, de Doelen Conference Center

Plenary Session Chairpersons: Professors Nicole Pamme and Thomas Laurell


Amy  ShenKeynote Presentation

Glioma on Chips: Analysis of Glioma Cell Guidance and Interaction in Microfluidic-Controlled Microenvironments Enabled by Machine Learning
Amy Shen, Professor, Okinawa Institute of Science and Technology, Japan

In biosystems, chemical and physical fields established by gradients guide cell migration, which is a fundamental phenomenon underlying physiological and pathophysiological processes such as development, morphogenesis, wound healing, and cancer metastasis. Cells in the supportive tissue of the brain, glia, are electrically stimulated by the local field potentials from neuronal activities. How the electric field may influence glial cells is yet fully understood. In this talk, I will present some recent research activities in my group to probe how electric fields in the microenvironment affect the migration of glioblastoma cells by using a versatile hybrid microsystems. To accurately analyze label-free cell migration, a machine learning software, Usiigaci, is developed to automatically segment, track, and analyze single cell movement and morphological changes under phase contrast microscopy. The hybrid microfluidic tools can enable us to elucidate fundamental mechanisms in the field of the tumor biology and regenerative medicine.


Lorena DiéguezKeynote Presentation

Using Microfluidics for Non-Invasive Cancer Diagnosis
Lorena Diéguez, Leader of the Medical Devices Research Group, INL- International Iberian Nanotechnology Laboratory, Portugal

Microfluidics presents numerous advantages for the handling of biological samples, as it provides careful control of fluids in the microscale. When it comes to biomarkers enrichment, microfluidics has demonstrated superior sensitivity and enhanced recovery compared to traditional methods. Incorporating sensors, lab-on-a-chip technologies offer efficient characterization of disease biomarkers from body fluids, making microfluidics ideal for clinical practice, enabling high throughput, portability, and automation. Early dissemination of cancer is difficult to detect by traditional imaging and pathological methods. While the presence of cancer material in body fluids is well known, current techniques for the isolation, analysis and characterization of these biomarkers are not efficient enough to be fully applied in clinical routine. In this talk, we present our work for isolation and multiplex analysis of cancer biomarkers from body fluids based on microfluidics, and biosensors towards personalized medicine and earlier diagnosis of cancer.


Robert VriesKeynote Presentation

Novel Organoid Models to Develop Drug Treatment Strategies
Robert Vries, CEO, Hubrecht Organoid Technology (HUB), Netherlands

Organoids such as IPSC derived brain organoids (Lancaster et al Nature 2013) or our adults epithelial stem cell derived organoids (Sato et al., Nature 2009, 2011) are proving to be a major breakthrough in preclinical models. The new patient like models are fundamental change in the way drug discovery and development can be performed. The development of the HUB Organoids started in the lab of Hans Clevers with the discovery of the identity of adult stem cells in human epithelial tissues such as intestine and liver (Barker et al., Nature 2007; Huch et al., Nature 2013). With the identification of these stem cells, we were able to develop a culture system that allowed for the virtually unlimited, genetically and phenotypically stable expansion of the epithelial cells from animals including humans, both from healthy and diseased tissue (Sato et al., Nature 2009, 2011; Gastroenterology 2011; Huch et al., Nature 2013, Cell 2015; Boj et al., Cell 2015).

We have now generated HUB organoid models from most epithelial organs. Recently, we and others have demonstrated that the in vitro response of organoids correlates with the clinical outcome of the patient from which the organoid was derived (Dekkers et al., Sci Trans Med 2016; Sachs et al., Cell 2018; Vlachogiannis et al., Science 2018). In addition, we have developed a coculture system using HUB Organoids and the immune system to study this interaction and drugs that target the role of the immune system in cancer and other diseases.

We have recently developed new models to study intestinal and lung barrier function and transport of the epithelium of these organs. These experiments show how organoids can be used to study mechanism that underly barrier function disruption in IBD or COPD. Furthermore, we have developed new models to study the interaction between immune system and epithelium. The combination of the new coculture models and assay development to study the epithelium allows us new insights into disease mechanisms and drug treatment strategies.


Morning Coffee Break and Networking in the Exhibit Hall


Valérie TalyKeynote Presentation

Droplet-based Microfluidics for Cancer Research
Valérie Taly, CNRS Research Director, Group leader Translational Research and Microfluidics, University Paris Descartes, France

Droplet-based microfluidics has led to the development of highly powerful tools with great potential in High-Throughput Screening where individual assays are compartmentalized within aqueous droplets acting as independent microreactors. Thanks to the combination of a decrease of assay volume and an increase of throughput, this technology goes beyond the capacities of conventional screening systems. Added to the flexibility and versatility of platform designs, such progresses in the manipulation of sub-nanoliter droplets has allowed to dramatically increase experimental level of control and precision. The presentation will aim at demonstrating through selected example, the great potential of this technology for biotechnology and cancer research. A first part of the presentation will exemplify how microfluidic systems can be used to compartmentalize and assay various types of cells without deleterious effects on their viability within complex and controlled platforms. The application of microfluidic systems for different cell-based assays will be demonstrated. Illustrative examples of droplet-based microfluidic platforms with high potential impact for cancer research will be presented. We will also show how by combining microfluidic systems and clinical advances in molecular diagnostic we have developed an original method to perform millions of single molecule PCR in parallel to detect and quantify a minority of target sequences in complex mixture of DNA with a sensitivity unreachable by conventional procedures. To demonstrate the pertinence of our procedures to overcome clinical oncology challenges, the results of clinical studies will be presented.


Emmanuel DelamarcheKeynote Presentation

Self-Coalescing Flows: A Powerful Method For Integrating Biochemical Reactions In Portable Diagnostic Devices
Emmanuel Delamarche, Manager Precision Diagnostics, IBM Research - Zürich, Switzerland

Diagnostics are ubiquitous in healthcare because they support prevention, monitoring, and treatment of diseases. Specifically, point-of-care diagnostics (POCDs) are particularly attractive for identifying diseases near patients, quickly, and in many settings and scenarios. POCDs can also trace exposure and acquired immunity of populations exposed to infectious diseases and screen metabolic deficiencies of individuals, who may be exposed to severe drug side effects. However, a long-standing challenge with POCDs is the need to integrate reagents in closed devices for a large number of potential applications. Following our previous contributions on developing capillary-driven microfluidic chips for highly miniaturized immunoassays, controlling and monitoring flow with nanoliter precision, and securing diagnostics against counterfeiting with dynamic optical security codes, we recently demonstrated how to shape and fold liquids inside microfluidic chambers to dissolve reagents with extreme precision. In this presentation, I will explain the underlying concept of this method, called self-coalescing flows, and will illustrate how it can be used to perform various assays, ranging from enzymatic assays, to immunoassays and molecular assays. Despite self-coalescing flows being still an open research topic in fluid physics, their implementation is surprisingly facile and robust and therefore may benefit the entire community working on POCDs.


Regina LuttgeKeynote Presentation

Nervous Systems-on-a-Chip: From Technology to Applied Biomedical Sciences
Regina Luttge, Professor, Eindhoven University of Technology, Netherlands

Challenges in eavesdropping on the complex cell signaling of the human central nervous system is an essential driver for the development of advanced in vitro technologies, called Brain-on-a-Chip. Developments in Brain-on-a-Chip technology focus primarily on the implementation of cortical cells from human stem cell source in a 3D cultured microenvironment. The aim of a recently launched EU project CONNECT is to mimic the in vivo functions of the nervous system in one connected chip system. The creation of new neurodegenerative disease models in this project brings together the knowledge accumulated among neuroscientists, stem cell experts and engineers to investigate the origins and possible treatments for Parkinson's disease. In this presentation, we will discuss in detail the technical approach of a nervous system on a chip as a unique tool for modelling the neural pathway of connected tissues on the brain-gut axis. In addition to design criteria for these microliter-sized physiological cell culture systems, the presentation will focus on guidance of the growth process of axon protrusions and the local control of cell differentiation processes while maintaining physiological conditions.


Networking Lunch in the Exhibit Hall, Exhibits and Poster Viewing

Session Title: Technologies and Applications in the Organs-on-Chip Space


The Glomerulus-on-a-Chip as a Platform for Disease Modeling, Drug Screening, Biomarker Discovery and mechanistic Studies
Stefano Da Sacco, Assistant Professor of Urology, GOFARR Laboratory for Organ Regenerative Research and Cell Therapeutics in Urology, Keck School of Medicine – University of Southern California, United States of America

Within the kidney, the glomerulus is the structure in charge of the renal ultrafiltration. Loss of glomerular function leads to renal damage and could end with irreversible damage. The major roadblocks to advancing new drugs and therapeutics designed specifically to preserve glomerular function stem from the inability to effectively develop multicellular in vitro models that can accurately mimic the architecture of the glomerular filtration barrier. We have recently developed a barrier-free, human based glomerulus-on-a-chip system that closely replicates the glomerular filtration barrier and its functions. In this session we will present our platform and discuss our newer insights on modeling of various diseases including membranous nephropathy, Alport Syndrome and diabetic nephropathy as well as describe potential applications ranging from mechanistic studies, biomarker discovery and personalized medicine.


Séverine Le GacKeynote Presentation

Organ-on-a-Chip Platforms for Assisted Reproductive Technologies
Séverine Le Gac, Professor, Applied Microfluidics for Bioengineering Research, University of Twente, Netherlands

Organ-on-a-chip platforms are currently considered as the next-generation in vitro models for various fields of applications such as drug and toxicity screening, disease modeling, tissue regeneration, metabolic studies, etc. Key-advantages offered by these platforms compared to standard in vitro models are, for instance, the possibility to accurately control the cellular microenvironment and to implement dynamic culture conditions in a microfluidic format, to emulate the architecture and/or the function of targeted organs by combining specific microstructures with cells, and to stimulate the cells in the device, e.g., chemically, mechanically, electrically, with an excellent spatiotemporal control. In my presentation, I will present current work from our group in the field of organ-on-a-chip with a particular focus on the field of assisted reproductive technologies (ART), with devices for the in vitro culture of mammalian embryos, the in vitro production of bovine embryos (oviduct-on-a-chip platform) and the ex vivo culture of testis tissues.


Developing Novel High-Throughput Organ-on-a-Chip Tissue Models for Drug Discovery and Unlocking Their Full Potential Using High-Content Imaging and Analysis
Silvia Bonilla García, Scientist, MIMETAS B.V., Netherlands
Kenneth Seistrup, Application Scientist, Molecular Devices, Denmark

In this presentation, scientific experts from MIMETAS and Molecular Devices will introduce the culture and interrogation of complex 3D tissues models in the OrganoPlate®, a unique 3D organ-on-a-chip platform, including models for gut-on-a-chip, angiogenesis and immuno-oncology. We will show examples of Organ-on-a-Chip tissues for use in disease modeling and drug safety testing and will present the new OrganoPlate® Graft, which allows vascularization of spheroids, organoids and PDX explants, and offers a powerful solution for the study of angiogenesis, an important focus for cancer therapeutics. These complex 3D tissue cultures can be interrogated in a high-throughput manner using powerful cellular imaging and analysis systems. We will show how Molecular Device’s ImageXpress imaging systems play a vital role in the development and analysis of 3D tissue models, such as those built on the OrganoPlate® platform, by enabling translatable assays and readouts for healthy and diseased human organ models as well as quantitative characterization of complex phenotypic effects.


Afternoon Coffee Break in the Exhibit Hall


Peter ErtlKeynote Presentation

Joint-on-a-Chip: Monitoring the Onset and Progression of Inflammatory Responses in a Personalized Rheumatoid Arthritis Model
Peter Ertl, Professor of Lab-on-a-Chip Systems, Vienna University of Technology, Austria

Rheumatoid arthritis is an autoimmune disease that causes inflamed joints through recruitment of immune cells, synovitis as well as degradation of cartilage. As a result, patients suffer from intense joint pain, are limited in their daily activities and have a lower life expectancy. To date almost 1% of the population worldwide is affected by arthritis, but no cure is available and pain reduction remains the treatment of choice. Although a number of drugs have shown to decrease disease progression, remission is achieved in less than 50% of patients, indicating a patient-specific drug response. Consequently, a personalized disease model is urgently needed to identify suitable drug combinations and concentrations for each individual patient. In this presentation the development of a sensor-integrated joint-on-a-chip platform capable of mimicking the physiological environment of a human diarthrodial joint is presented and observed patient variability discussed.


Blood Vessels-on-Chips
Andries D. van der Meer, Tenure Track Assistant Professor, Faculty of Science and Technology, University of Twente, Netherlands

The total surface area of blood vessel wall in the human body is more than 1,000 m2, equal to more than five tennis courts. The vessel wall is not simply a physical barrier between blood and surrounding tissue, it is also a remarkably plastic structure, which plays a key role in the pathophysiology of many diseases. Organs-on-chips are ideal platforms to model blood vessel biology in vitro, because they allow researchers to set defined and dynamic patterns of flow, pressure, geometry and levels of oxygen, all of which are essential aspects of blood vessel physiology. Moreover, organs-on-chips enable the controlled co-culture between blood vessel tissue and neighboring tissues and matrices, ultimately allowing studies of disease mechanisms. In this talk, I will show how blood vessel tissue, primarily endothelial cells, can be integrated in organs-on-chips to realistically mimic blood vessel physiology. Moreover, I will show how exposing these blood vessels to activating stimuli, like inflammatory cytokines, can induce vascular adhesion molecule expression, vascular leakage and thrombosis. Finally, I will discuss how organ-on-chip technology allows the engineering of ‘personalized’ in vitro models of blood vessels by mimicking 3D vessel structures based on medical imaging, perfusion of blood samples spiked with disease-related cytokines and incorporation of vascular tissue derived from human induced pluripotent stem cells.


Networking Reception with Beer and Wine in the Exhibit Hall -- Meet Exhibitors, View Posters and Network with Colleagues


Close of Day 1 of the Main Conference

Thursday, 10 September 2020


Morning Coffee and Networking in the Exhibit Hall

Session: Organoids, Organ-on-a-Chip and Tissue-on-a-Chip -- Where are these Fields Heading?


Guided Tissue Assembly Biofabrication of Epithelial Organoid Tubes
Yan Yan Shery Huang, Lecturer, University of Cambridge, United Kingdom

Epithelial organoids cultured in appropriate 3D conditions typically develop a microscopic, cystic structure lined with a polarized epithelium. Despite their great potential in research and therapy, epithelial organoids grow in heterogeneous sizes, and are too small to display physiologically relevant performance and applications. Here, we show guided assembly of mouse tracheal basal stem cell organoids towards geometrically-defined, lumenized constructs. Drawing parallel to liquid droplet interaction, we provide hypothesis on how epithelial organoid assembly can be achieved in a more efficient and predictable manner, of which principles could be extended to other organoid types developed form epithelial stem cells. The guided self-assembly strategy presented here opens up the possibility for biofabricating size-relevant, geometrically defined epithelial structures towards broad applications in biomimetic organoid-devices and tissue engineering.


CELLINK3D-Bioprinting Technologies For Tissue Engineering and Organ-on-chip Development
Pierre-Alexandre Laurent, Senior Field Application Specialist, CELLINK

We will present how our 3D bioprinting technologies contribute to the tissue engineering, the organoids research and the organ-on-chip development. We will discuss how CELLINK’s technology enables the large-scale manufacturing of tissue constructs, which have wide application, including the development and validation of the efficacy of pharmaceutical and cosmetic products, screening of drug therapies, understanding of tissue development and oncology processes, and in the future translation to use in clinical applications. In addition, we will introduce our bioinks, which enable researchers to culture human cells to grow into functional tissue constructs. They provide a tissue specific microenvironment that cells can thrive in and allow to tailor a complex multicellular structure furnishing features of functional organs. Finally, we will discuss how cell biology and 3D-bioprinting approaches, are used to print organoids and tissue structures (such as liver, heart, skin), functional cancer tumor models as well as perfusable vascular network.


Modeling Immune Mediated Beta Cell Destruction in Human Type 1 Diabetes with Organoids
Matthias von Herrath, Vice President and Senior Medical Officer, Novo Nordisk, Professor, La Jolla Institute, United States of America

In the past 15 years we have been studying the pathology of human type 1 diabetes with access to donor pancreata through the human pancreatic organ donor consortium (nPOD). These studies have led to several findings, for example that certain cytokines are generated by beta cells themselves, sometimes under stress, and also that there are probably key factors that render beta cells susceptible to immune attacks. Mechanistically, the importance and meaning of these observations needs to be addressed in a suitable and easily manipulable in vitro system consisting of human islets and immune cells. We have built such a system in collaboration with the company InSphero and will discuss emerging findings.


Morning Coffee Break and Networking in the Exhibit Hall


Microfluidic ChipShopIt’s the Economy – Industrial Aspects of Organ-on-a-Chip Device Manufacturing
Holger Becker, Chief Scientific Officer, Microfluidic ChipShop

While academic activities in the organ-on-a-chip field have multiplied in recent years, it becomes apparent that translating academic results into commercially viable products can be challenging. This is even more true for such devices which require a generically multidisciplinary approach, combining application know-how with surface chemistry, microfabrication and materials technology. In this presentation, we will give an overview over available solutions for such products and explain classical pitfalls on the way from the academic laboratory bench to an industrial product.


Microfabrication Technologies For Engineering a Joint on Chip
Marcel Karperien, Professor, University of Twente, Netherlands

Osteoarthritis (OA) is a degenerative joint disease affecting more than 130.000.000 patients world-wide. The etiology of the disease is still poorly understood. While initially considered as a disease of cartilage it is now clear that the disease involves all tissues in the joint. A disease trigger in each of these tissues can initiate the onset of disease and each of these triggers converge over time in a similar disease presentation. Despite tremendous efforts in the recent past, the disease can still not be treated.  It is believed that lack of translational power of currently used in vitro and in vivo animal models can at least in part this lack of success. It has also become clear that currently used animal models poorly reflect the complexity of human disease nor do they allow the detailed study of the complex interactions between the different joint tissues at various stages of disease. To address these shortcomings my group has started the development of a joint-on-chip. The joint-on-chip has a modular chip design (Piluso et al., 2019). We are engineering chips for each individual tissue, i.e. cartilage, subchondral bone, synovium, ligament and/or meniscus which together form the joint. The individual chips are connected to each other through blood vessel mimicking microfluidic channels  as well as with a chip mimicking the intra-articular space. This latter chip will contain features allowing non-invasive imaging / sensing of inter tissue communication. Since movement is a critical and an essential feature in every joint, the individual chips can all be independently actuated mimicking both compression and shear stress. Prototype chips of the synovium and the cartilage including actuation have become available (Paggi et al., 2020). Additionally we developed various strategies for introducing cell laden membranes composed of natural polymers and/or tissue constructs in the chip. Finally, we have developed sensors that can assess local matrix metalloproteinase activity, a key factor driving joint degeneration on chip. In my presentation I will discuss various engineering aspects of our microfabrication technology platforms needed for recreating a representative and functional human joint. It is expected that these efforts will help us studying the pathophysiology of osteoarthritis and will accelerate the development of dearly needed osteoarthritis disease modifying treatments.


George TruskeyKeynote Presentation

Vascular Human Microphysiological Systems
George Truskey, R. Eugene and Susie E. Goodson Professor of Biomedical Engineering, Duke University, United States of America

Microphysiological systems (MPS) are micron scale three-dimensional models of key structural or functional units of human organs or tissues.  Such systems can be used to model diseases and test therapeutics.  Models of the vascular system have examined both microvascular networks and large blood vessels. The strengths and limitations methods to produce microvascular and arteriole-scale MPS will be described.  Human cells sources for endothelium and medial cells will be considered, including the use of induced pluripotent stem cells (iPs) to produce function endothelium and smooth muscle cells.  Applications of these systems to model genetic and acquired diseases and evaluate therapeutics will be described.


Danilo TagleKeynote Presentation

The NIH Tissue Chips Program and Deployment in Emerging Health Crises
Danilo Tagle, Associate Director For Special Initiatives, Office of the Director, National Center for Advancing Translational Sciences at the NIH (NCATS), United States of America


Thomas LaurellKeynote Presentation

Title to be Confirmed.
Thomas Laurell, Professor, Lund University, Sweden


Networking Lunch in the Exhibit Hall and Networking with Exhibitors


Poster Viewing Session


InTESTine Barrier Chip: A Microfluidic ex vivo Model to Study Intestinal Permeability and Host-Microbe Interactions in the Human Intestinal Tract
Hossein Eslami Amirabadi, Scientist, TNO, Netherlands

We developed a microfluidic chip, InTESTine Barrier Chip, with a 3D clicking mechanism that fixes intestinal biopsies between two microfluidic channels. This novel technology enables us to study intestinal processes such as drug absorption and immune responses.


A Novel System of Infusion to Provide Drugs Within ex vivo Skin Models
Emma Raude, Researcher, LAAS-CNRS/Genoskin, France

We characterize a novel microfluidic system of infusion to provide high molecular weight molecules within ex vivo skin models. The results position the system as a new relevant tool to study the efficacy and/or toxicity of intravenously administered biotherapeutics directly on human skin.


Elisabeth VerpoorteKeynote Presentation

Title to be Confirmed.
Elisabeth Verpoorte, Professor of Analytical Chemistry and Pharmaceutical Analysis, University of Groningen, Netherlands


A Biomimetic Human Gut-on-a-Chip For Oral Drug Delivery Testing
Nayere Taebnia, Researcher, Technical University of Denmark, Denmark

This presentation introduces a biomimetic human gut-on-a-chip platform, fabricated using stereolithographic high-resolution 3D printing, which recapitulates the complex geometry of the native tissue and advances our understanding of intestinal physiology and its role in drug uptake and personalized medicine.


Christine MummeryKeynote Presentation

Closing Keynote
Christine Mummery, Professor and Chair of Developmental Biology, Leiden University Medical Centre, Netherlands


Close of Conference

Add to Calendar ▼2020-09-09 00:00:002020-09-10 00:00:00Europe/LondonOrgan-on-a-Chip, Tissue-on-a-Chip and Organoids Europe 2020Organ-on-a-Chip, Tissue-on-a-Chip and Organoids Europe 2020 in Rotterdam, The NetherlandsRotterdam, The