Abstract

The Identification of a Long Stress-Induced Non-Coding Transcript which has Increased Expression in Breast and Ovarian Cancers

David Smith, Professor, Mayo Clinic

Using whole genome tiling arrays we sought to identify long non-coding transcripts which had altered expression in response to the major carcinogen in cigarette smoke. We therefore took a normal bronchial epithelial cell line and exposed it to NNK and then characterized any potential non-coding transcripts that had increased expression after this exposure. This led to the identification of 12 long non-coding transcripts which were referred to as Long Non-Coding Stress Induced Transcipts (LSINCTs). One of these, LSINCT5 had increased expression in most breast cancer cell lines and primary tumors analyzed. We characterized this long non-coding transcript further and determined its’ full size, its cellular localization and its expression in both breast and ovarian cancers. This transcript did indeed have increased expression in most breast and ovarian cancers. We further determined that this transcript was responsible for inducing cellular growth and that it appeared to co-localize with the nuclear paraspeckles. We will describe our work on this long non-coding transcript.