Abstract

96 Kidney Tubules On-a-Chip For Real-Time Toxicity Testing

Paul Vulto, Managing Director, MIMETAS

Drug toxicity remains a major issue in drug discovery and stresses the need for better predictive models. Here, we describe the development of a perfused renal proximal tubule cell (RPTC) model in Mimetas’ OrganoPlates™to predict kidney toxicity. The OrganoPlate™ is a microfluidic platform, which enables high-throughput culture of boundary tissues in miniaturized organ models. In OrganoPlates™, extracellular matrix (ECM) gels can be freely patterned in microchambers through the use of PhaseGuide™ technology. We demonstrate 96 perfused RPTCs on one OrganoPlate on which toxicity effects can be monitored in real-time. The model capitulates crucial aspects of the proximal tubule, including leak-tightness, apical-basal polarization and expression of a range of crucial transporters.