Abstract

Integrating Advanced Fluorescence Microscopy Techniques Reveals Nanoscale Architecture And Mesoscale Dynamics Of Cytoskeletal Structures Promoting Cell Migration And Invasion

Alessandra Cambi, Professor, Radboud University Medical Center

Podosomes are dynamic integrin- and actomyosin-based multi-molecular adhesion structures involved in cell protrusion, topography sensing and extracellular matrix degradation in a variety of cells. With an actine core of ~0.5um, hundreds of podosomes organize in different mesoscale spatial arrangements ranging from large circular belts in bone-degrading osteoclasts to small rosettes in endothelial cells and well-defined large clusters in antigen-presenting cells. The molecular mechanisms regulating formation and maintenance of these mesoscale arrangements are still poorly defined.