Abstract

An Improved Method for Sensitive and High-throughput Quantification of cfDNA in Liquid Biopsy using the Clarity Tube-strip Digital PCR

Johnson Ng, Founder And Chief Executive Officer, JN Medsys Pte Ltd

Digital PCR is one of the fastest growing technologies for genetic analyses. Sample partitioning, where a PCR sample is divided into a large number of partitions such that each contains only 0 or 1 target DNA copy, is an important step in digital PCR. However sample partitioning is technically complex to achieve. Here, we introduce tube-strip digital PCR on the Clarity system. Sample partitioning requires just 1 min and is performed on a high-density chip within a conventional PCR tube-strip. Workflow is fast and easy, with the flexibility to perform up to 96 reactions per run. We show how the Clarity digital PCR system is an attractive platform for performing for sensitive and high-throughput quantification of cell-free DNA in liquid biopsy.