Abstract

Addressing the Challenges to Quantitative High Resolution, High Throughput Confocal Imaging of 3D Spheroids

Jeffrey Morgan, Professor of Medical Science and Engineering, Brown University

It is now widely recognized that new, more predictive in vitro assays are needed for drug discovery and toxicity testing. 3D spheroids offer far more biological complexity and content than cells grown in 2D conventional plates. But before this content of spheroids can be accessed, significant challenges to imaging, especially at higher magnification, must be overcome. This talk will discuss some of these challenges and focus on a new high throughput method for the formation and long term culture of spheroids, the formation of designer spheroids as well as the use of high throughput confocal microscopy for live cell imaging in spheroids.