Abstract

Microfluidic Organ-on-a-Chip Devices for Stem-Cell Cultivation, Differentiation and Toxicity Testing

Holger Becker, Chief Scientific Officer, microfluidic ChipShop GmbH

We have developed diagnostic multi-organ microfluidic chips based on patient specific induced pluripotent stem cell (iPS) technology to explore liver dependent toxic effects of drugs on individual human tissues such as liver or kidney cells. Based initially on standardized microfluidic modules for cell culture, we have developed integrated microfluidic devices which contain different chambers for cell/tissue cultivation as well as on-chip cell-culture medium actuation with an on-board peristaltic pump and gas exchange using integrated membranes. The devices are manufactured using industrial manufacturing methods. In the project, suitable surface modification methods of the used materials as well as hybrid integration of different elements had to be explored. We have been able to successfully demonstrate the seeding, cultivation and further differentiation of modified iPS, as shown by the use of differentiation markers, thus providing a suitable platform for toxicity testing and potential tissue-tissue interactions.