Abstract

Nano-Based Platforms For Controlling Neuronal Organization and Growth

Orit Shefi, Associate Professor, Bar-Ilan University

In this talk I will present our recent studies of 3D nanostructured platforms for promoting and directing neuronal growth and drug delivery. As physical mechanical forces and contact guidance play key roles in neuronal morphogenesis, we used magnetic nanoparticles (MNPs) as mediators to apply forces locally on neurons throughout their migration. Following incubation, MNPs accumulated in the cells, turning the cells sensitive to magnetic stimulation. Then, we applied magnetic fields with controlled magnetic flux densities at multiple scales of size and strength. Plating the cells atop a magnetic micropatterned device has led to an organized network of cell clusters. Next, we used the ability to control MNP movement to shape the extracellular environment of the cells. We mixed MNPs in collagen hydrogel and applied external magnetic field during the gelation period, aligning the MNPs and the collagen fibers. As neurons rely on physical topographical elements, neurons embedded within the aligned gel demonstrated polarized growth pattern. This platform is now examined as a novel method to direct neuronal growth and to bridge gaps efficiently post trauma. Our study presents an emerging magneto-chemical approach for promoting neuronal growth and repair.