Abstract

Oral Talk: Blue-Light Accelerated Formation of Bio-mediated or Coated Silver and Gold Nanoparticles and their Promising Applications

Kunzes Dolma, PhD Scholar , IMTECH

Functionalized nanomaterials has opened a whole new range of versatile opportunities for innovative applications in widespread sectors of life ranging from small electronic devices such as biosensors to drug delivery, medical and disease diagnostics1. However, challenge still remains with biomaterial functionalization on the nanoparticle due to various factors necessary to sustain a functional biological entity. Currently, formulating a functionalized nonmaterial involves three critical steps, first, synthesizing a stable chemically derived nanoparticle, secondly, modification of surface using a functional group (thiol or other groups) and finally capturing the protein on the chemical group present on the surface of nanoparticle1. Functionalization process generates uniform and controlled coating of surface molecule, but it is a multi-step process. These three step processes can be reduced to one step if we can optimize the conditions such that it is suitable for biomaterial to form nanoparticle by itself. To date very few biomaterials have been reported to form nanoparticle or nanoclusters such as proteins (BSA, lysozyme, transferrin etc), peptides, DNA and carbohydrates also2-7. However, their rate of formation is considerably slow and more importantly very high concentration of biomaterials is required to form the nanoparticle. People have not explored exotic proteins and mostly the commonly available or so called model proteins are subjected to this kind of studies. Moreover, conditions optimized for one protein may or may not be applicable for other proteins. So, one pot synthesis of nanoparticle by proteins has not been successful to date. Here we report role of light, particularly blue light which enhanced formation of both silver and gold nanoparticle by several different proteins/peptides and small molecules. We show here that light dominates as a seed factor much more than protein identity, buffer condition and molar ratio of protein. Further, the present study also confirmed that the biomaterials coated nanoparticles also retains its function of binding to its natural binding partners. I addition the cytotoxicity studies in different types of cell lines also confirmed that the biomaterials coated nanoparticle are comparatively non-toxic.