Abstract

Microfluidic Chip System Combined with Tetrahedron DNA Nanostructures For Circulating Tumor Cell Capture

Xianqiang Mi, Professor, Chinese Academy Of Sciences

As the typical liquid biopsy technology for cancer prevention and control, circulating tumor cells (CTCs) analysis shows great prospects. However, the extremely low content of CTCs in blood brings great difficulty for its capture and detection. Microfluidic chip technology and DNA nano technology have made great progress in CTCs capture, but there are still some shortcomings, such as low capture efficiency, narrow spectrum for target and easily leading to cell inactivation. Here, based on the principle of microfluidic immuno-affinity, we propose a new CTCs capture method with the combination of microfluidic chip and DNA nanostructure technology. The performance of the developed microfluidic chip was verified by the tumor cell suspension and real blood samples. This new CTC capture method has the advantage of high capture efficiency, broad spectrum for target and good ability to keep cell viability.