Abstract

Revolutionizing Single Cell Research with High Throughput Single Cell RNA-Seq

Muriel Breteau, Senior Technical Applications Specialist, Dolomite Bio

Analyzing single cells has enabled researchers to understand the complexity of biologic systems and gain vital information and data which is key to understanding many diseases and immunity. However, until recently, methods involved bulk analysis, causing vital information at the single cell level to be lost.

With the emergence of scRNA-Seq, this has offered an exciting, cutting edge technique that for the first time gives unprecedented insight into the expression of thousands of single cells. This method has revolutionised the way we analyse single cells and enables additional understanding into tissues at the level of individual cells.

This presentation will investigate:

• Current methods to perform single cell RNA-Seq including the effective use of microfluidic droplet technology to enable access to the transcriptome of thousands of single cells in a high throughput manner
• Advantages and benefits of using the drop-seq protocol for scRNA-Seq
• The development of new protocols and application using flexible single cell tools including profiling natively paired TCRs, isolating antibody coding sequences, encapsulating cells in hydrogels or encapsulating expression libraries for FACS sorting