Abstract

Towards Single-cell DNA Digital Sequencing

Larry Xi, Chief Scientific Officer, Digenomix Corp

Single-cell DNA sequencing has been shown to gain greater insights into the clonal structures of heterogeneous cancer tissues that bulk DNA sequencing has never been able to resolve.  However, biased whole genome amplifications from single-cell DNA reduce the resolution and the potential of this approach.   We proposed a scheme, BIG (Barcoding-In-Genome), that could increase the resolution by turning the analog mode of DNA sequencing into digital mode.  This digital mode will give exact, absolute copy number for every gene and can only be achieved on single-cell platforms.