Abstract

High Content Screening of an Antigen-Specific 3D Tumor-Spheroid/Primary T-cell Co-Culture System

Shane Horman, Research Investigator, Genomics Institute of the Novartis Research Foundation

Overcoming tumor-mediated immunosuppression and enhancing cytotoxic T-cell activity within the tumor microenvironment are two central goals of immuno-oncology (IO) drug discovery initiatives. Here we present a novel ultra-high-content assay platform for interrogating antigen-dependent T-cell-mediated killing of 3D tumor spheroids that yields multi-parametric, clinically-relevant data and can be employed kinetically for the discovery of novel IO therapeutic agents.