Abstract

HCS Advances in 3D Cellular Imaging

Joe Trask, Senior Field Applications , Perkin Elmer

The recent emergence of more relevant advanced and complex in vitro cell model systems to recapitulate an in vivo response requires new techniques and tools to interrogate these 3D cell model systems.  High content imaging is an ideal and proven technology to provide tens to hundreds of multiparameter feature data to phenotype subpopulations and to identify spatial measurements at the single cell level to determine outcomes following chemical exposure or drug treatment screening.  In this talk I will discuss the challenges from imaging these cell models (organoids, spheroids, microtissue, and organ-on-a-chip) and provide solutions for image analysis as a guideline to better understand these complex 3D HCI data that includes strategies for developing robust assays that are reproducible.