Abstract

Single Cell Array Printing Technique and Its Application for Cancer Migration

Shengli Mi, Professor, Tsinghua University-Shenzhen

Single-cell arrays are powerful tools for many biological processes such as cellular behavioral analysis, cancer development, cell-cell communications, and drug screening and metabolism. Based on the Alternating Viscous-Inertial Force Jetting, we present a novel single-cell printing technique for the construction of single-cell arrays on microfluidic chips. First of all, this work accomplished the construction of single-cell printing platform and parameters optimization. Then a pattern microfluidic device matched with single-cell array was proposed to verify the pattern control capability of the single-cell printing platform and the effectiveness of the printing process. Finally our work come up with another microfluidic chip which had hundreds of micro-reactors and studied the migration ability and high-throughput anti-cancer drug responses of cancer cell as an important application.