Abstract

Biophysical Approaches To Organoid Fabrication

Kennedy Okeyo, Senior Lecturer, Institute for Frontier Life and Medical Sciences, Kyoto University

Organoid generation by biochemical induction strategies faces scalability challenges owing to cost implications stemming from the use of costly reagents. On the other hand, biophysical techniques relying on modulating the cell culture milieu to induce self-assembly, direct differentiation, and, ultimately, organoid formation presents a viable alternative approach with ease of scalability, higher reproducibility and cost effectiveness. Here, we introduce such a biophysical technique, namely, the mesh culture technique, and demonstrate its potential in directing self-assembly and differentiation of stem cells. Ongoing efforts to integrate this technology with microfluidics to realize organoid-on-a-chip system for drug assay and biological studies will be presented.