Abstract

Ultra-Sensitive Mutational Analysis in Cell-Free DNA by Digital PCR and NGS Technologies

Rachel Tam, Senior Scientific Researcher, Genentech

Circulating cell-free DNA (cfDNA) in plasma offers a non-invasive approach to monitor tumor molecular profiling in real-time at multiple time-points, detection of emerging genomic alterations associated with drug resistance and clarifying cancer prognosis and diagnosis of cancer recurrence or progression. We developed an ultra-sensitive droplet digital PCR (ddPCR) approach and molecular tagged NGS technologies to detect actionable cancer biomarkers in cfDNA.