Abstract

Routine Single EV Protein Biomarker Analysis

Clayton Deighan, North American Sales and Applications Manager, NanoFCM

One of the major limitations to the realization of the potential in EV biomarkers is the lack of techniques for phenotyping individual EVs. In this presentation we will present the ExoView platform, a new tool for the capture and characterization of EVs from many sources. By coupling a validatable immunoassay for capturing EVs with no prior purification with a sensitive interferometric measurement of single EVs (50+nm) with no additional labeling we separate important populations from the noise.  The secondary analysis by counterstaining for biomarkers of interest can be custom designed by the user allowing for detection of the markers of interest in their research with minimal assay development time.  Example datasets for marker colocalization will be presented for EVs from a variety of sources.