Abstract

Cancer Cell Spheroids as 3D Model For Drug Screening In Droplet-based Microfluidics

Mario Saupe, , Institut für Bioprozess- und Analysenmesstechnik e.V. Heiligenstadt

Cancer is one of the leading cause of deaths worldwide. Among others this is owed to limited therapeutic options caused by a high degree of resistances to conventional chemotherapeutics and an aggressive tumor progression. In order to overcome these resistances, new therapeutic targets, e.g. new drugs, have to be identified and investigated. In the last years a lot of microfluidic approaches were developed to improve therapeutic success after cancer diagnosis. To investigate the behavior of cancer cell spheroids under treatment with chemotherapeutics, appropriate cell viability assays is adapted to the droplet based microfluidics. In this presentation, we introduce the microfluidic technological platform and its functional modules as well as first results of the experiments using the alamarBlue®-viability assay.