Abstract

Selecting and Validating Fit-for-Purpose Assays to Interrogate 3D Culture Models

Terry Riss, Global Strategic Marketing Manager Cell Health, Promega Corporation

There is a rapid expansion in the use of 3D cell culture model systems ranging from individual scaffold-free spheroids to multiple organoids designed to represent a human-on-a-chip. Researchers soon become aware the spectrum of 3D models have vastly different culture requirements and there is no “one size fits all” approach. Selecting a 3D culture model that is “fit for purpose” often involves compromises considering sample throughput, complexity, physiological relevance, cost and limitations in the available assay technologies. I will describe an overview of factors to consider when designing an appropriate 3D culture model and stress the importance of considering limitations of assay methods to interrogate relatively large 3D structures.