Abstract

Mobile Diagnostics - Multiplexed DNA Malaria Sensing using Origami Paper Folding and Blockchain/Expert AI Digital Healthcare Systems

Jonathan Cooper, Wolfson Professor and University Vice Principal, Glasgow University

Identification of the species of pathogen is often key to informing the treatment of patients with infectious diseases. Here, we use paper-based folding, in a manner similar to origami, to process patient samples from a finger-prick of blood and diagnose malaria amongst communities in rural villages.   The design of the DNA sensing device means that testing can be done by a non-expert, integrating sample preparation into a multiplexed lateral flow device – akin to a conventional pregnancy test. We have tested our devices working with the Ministry of Health in Uganda, using a mobile phone to run the tests, collect and collate data and provide expert decision support for the healthcare workers. The technique has the potential to provide new diagnostic information that can direct therapy, thereby reducing the future threat of anti-microbial resistance.  The tests could also find applications in identifying asymptomatic carriers with low levels of infection, thereby supporting the delivery of disease elimination programmes.