Abstract

CTC Isolation for Single Cell Analysis

Clotilde Costa, Leader of the Liquid Biopsy Line, Joint Unit Roche-Chus, Oncomet, Universitary Cinical Hospital of Santiago de Compostela

CTCs analysis at single-cell level allows the study of tumor heterogeneity in a minimally invasive way. CTCs repeated sampling offers real time information of the tumor and potentially is a tool for therapeutic strategy decisions. Present research shows single cell isolation using three different technologies (VyCAP puncher, DEPArray and manual micromanipulation) and subsequent sequencing analysis (Oncomine comprenhensive assay). Metastatic breast and prostate cancer patients before and after treatment (chemotherapy) were considered for this study.