Abstract

Selection of Single Cells by Function Using Lab on a Particle Technology

Dino Di Carlo, Armond and Elena Hairapetian Chair in Engineering and Medicine, Professor and Vice Chair of Bioengineering, University of California Los Angeles

The ability to create picoliter and nanoliter compartments using microfluidics has driven biotechnology at its ultimate "quantum limit" of single cells and molecules.  I will discuss the progress we have made in developing assays for cell secretion and growth, leveraging particles to impart new features on compartments, such as ease of use or compatibility with production environments. I will discuss applications ranging from microalgal culture and clone selection to workflows for selecting antibody-secreting cells using standard fluorescence activated cell sorters. Lab on a particle technology promises to expand the capabilities and the accessibility of quantum biological assays.