Abstract

Customizing Single Cell Research

Thomas Ayers, Sr. Technical Applications Specialist, Dolomite Bio

Here we report a flexible and cost-effective droplet-based microfluidics system, called the Nadia Instrument, that can allow 3’ mRNA capture of ~50,000 single cells or individual nuclei in a single run. The precise pressure-based system demonstrates highly reproducible droplet size, low doublet rates and high mRNA capture efficiencies that compare favourably in the field. Moreover, when combined with the Nadia Innovate, the system can be transformed into an adaptable setup that enables use of different buffers and barcoded bead configurations to facilitate diverse applications. We demonstrate how this robust and versatile system unlocks high throughput single cell profiling for large plant leaf protoplasts and single cells or nuclei with poor RNA yields. With the Nadia Innovate, we present a solution for diverse sample types, whilst allowing researchers to complete more single cell transcriptomic projects within budget.