Abstract

YAMAHA CELL HANDLER: Automated, Rapid Spheroid, Organoid and Single Cell Sort System by Imaging and Picking

Yuichi Hikichi, General Manager, MDB Division , Yamaha Motor

By using various parameters of images, like size, shape and fluorescent intensity, the CELL HANDLER^TM can automatically select single cells or 3D spheroids and sort them to various types of culture plates. It uses a unique disposable tip to ensure the damage-free and accurate handling and enables direct picking of organoids from gel. Conventional manual methods have been hampered by low throughput and suboptimal accuracy. Conventional cell sorters damage cells and need significant amounts of cells and are not applicable for spheroids and organoids. The CELL HANDLER complements these deficiencies. It enables more accurate and higher throughput sorting than manual operation and low amounts of cells (e.g. 100-1000 cells) can be sorted without damages. Any types of plates (e.g. microtiter plates, micro cavity plates, 3D colony in gel, 2D colony on plate, organ-on-a-chip) can be used as a source plate and destination plate, which will support many types of applications. The CELL HANDLER is a high-speed, high-precision pick and place system coupled with image capture and analysis capability that greatly increases the efficiency and reliability of spheroid/organoid and single cell assays.