Abstract

Multi-Parametric High-Throughput Flow-Based Analysis of Single Extracellular Vesicles

Daniel Chiu, Professor, University of Washington

We have developed a multi-parametric high-throughput flow-based method for the analysis and sorting of individual extracelluar vesicles (EVs), which are highly heterogeneous and comprise a diverse set of surface protein markers as well as intra-vesicular cargoes. Yet, current approaches to the study of EVs lack the necessary sensitivity and precision to fully characterize and understand the make-up and the distribution of various EV subpopulations that may be present. In fact, most current EV isolation methodologies, including differential centrifugation, affinity/immuno-magnetic isolation, polymer-based precipitation, size-based exclusion, can be prone to contaminations and cannot resolve single EVs. Our new technology platform is able to immune-phenotype EVs and quantify proteins associated with EVs, at the single-EV level, with high precision and throughput.