Abstract

Improving Model Quality and Reliability by Combining Organoid Technology with Organ-on-a-Chip Systems

Peter Ertl, Professor of Lab-on-a-Chip Systems, Vienna University of Technology

Organoid technology and in particular embryonic stem cell (ESC) models are of great interest in biomedical research because they provide deeper insights into e.g. neurogenesis and early mammalian brain development. Despite their great scientific potential, the reliable establishment of size-controlled spheroids and three-dimensional embryoid bodies remains a major challenge. The current lack of standardization, robustness and comparability is further l limiting a broader application and translation of stem cell technology. To overcome these limitations a microfluidic biochip array was designed and employed to automate cell loading, establish size-controlled organoids and differentiation protocols using murine P19 embryoid bodies.