Abstract

EV Analysis Using Imaging Flow Cytometry

Stephanie Brunelle, Senior Product Manager, Luminex Corp.

Amnis flow cytometers leverage the superior photonic sensitivity provided by time delay integration charge coupled device (TDI CCD) cameras. High gain mode on the ImageStream® and small particle mode on the CellStream® systems further enhance that sensitivity making these systems ideal for measuring extracellular vesicles (EVs) and other small particles. With multiple excitation lasers and up to 20 fluorescent channels, EV experiments can include probes for cargo proteins, RNA, and other molecules when needed. In this presentation, we will review the life cycle of EVs, talk about the critical controls needed for EV experiments, and show data on particle detection sensitivity, cargo protein and RNA, and uptake of EVs by other cells.