Abstract

Digital Flow Cytometry For Single-EV Analysis

Daniel Chiu, Professor, University of Washington

We have developed a multi-parametric high-throughput flow-based method for the analysis of individual extracelluar vesicles (EVs), which are highly heterogeneous and comprise a diverse set of surface protein markers as well as intra-vesicular cargoes. Yet, current approaches to the study of EVs lack the necessary sensitivity and precision to fully characterize and understand the make-up and the distribution of various EV subpopulations that may be present. Digital flow cytometry provides single-fluorophore sensitivity to enable phenotyping single EVs with unprecedented precision and sensitivity.