Abstract

Improvements in fluorescent Nanoparticle Tracking Analysis (f-NTA): Reliable Characterization of Extracellular Vesicles

Sven Rudolf Kreutel, Sales Director, Particle Metrix GmbH

During the last decade, Nanoparticle Tracking Analysis (NTA) has emerged as a vital and fast characterization technology for Extracellular Vesicles, Exosomes and Microvesicles for concentration and size. While classic NTA scatter operation feeds back total particle concentration, the fluorescence detection capability enables the user to gain specific biochemical information for phenotyping of bio nanoparticles for estimation of sample purity. Here we report on latest improvements in size resolution and multi-channel fluorescent colocalization studies on standard material as well as selected biological vesicles.