Abstract

Microfluidic Chip For In Vitro Neuronal Cell Culture Under Electrical Stimulation

Ana Ayerdi , Senior Researcher, Tecnalia Research & Innovation

A chip with a fluidic design that allows neuronal cell culture and axon growth monitoring was developed. This design consists of two separated chambers in which cells are seeded and allows the monitoring of axons outgrowth through the microchannels. Using industrial compatible processes, we have fabricated polymer shims and working stamps to manufacture such patterned surfaces both in a small scale plate to plate fashion as well as continuous high throughput roll-to-roll fabrication. The material choice allows for fluorescence microscopy imaging. The design was screened in vitro with different neuronal like cell lines and using several substrates that can be used for high throughput roll-to-roll manufacturing. The design is also compatible with a custom-made electrical stimulus generator. Neuronal cells respond to electrical stimulation in two ways: by increasing their cell size and by increasing the cell number in culture. With this approach chips that can be manufactured in a faster and roll to roll fabrication method for in vitro drug screening assays will be shown.