Abstract

Know Your EVs Inside Out with ExoView

Min Kyoung Sun, Field Application Scientist, Unchained Labs

Comprehensively characterizing your EVs can be a struggle even in a highly purified cell culture sample. Throw in complex biofluids, limited sample volume and rare EV subpopulations and that struggle can become a nightmare. Thankfully the ExoView R200 provides the solution! We will discuss how our proprietary ExoView chips utilize the power of affinity capture to isolate EVs from starting materials ranging from cell culture media to murine cerebral spinal fluid. All without the need for prior sample purification. Once EVs are bound to the surface of the chip, the ExoView platform can size, count and phenotype vesicles on a single particle basis, in one sample run. The R200 features 4 fluorescent channels (blue, green, red and far red) allowing individual EVs to be phenotyped by up to 5 surface or cargo proteins with single molecule sensitivity. We will cover the use of ExoView Tetraspanin kits for providing EV characterization on the basis of common exosome markers and highlight how ExoFlex chips allow full user customization to enable capture and detection of EVs via specific cell or disease markers.