Abstract

New Flow-Cytometry Technologies: From Rare-Cell Isolation to the Analysis of Single Extracellular Vesicles and Particles

Daniel Chiu, Professor, University of Washington

This presentation will describe two flow-based technologies we developed for the analysis of single rare cells and individual extracellular vesicles and particles. The first flow platform is a rare-cell isolation instrument, which is capable of operating in a rapid sequential sorting mode for isolating single rare cells with exceptionally high sensitivity and purity. The second flow instrument is a digital flow cytometer, capable of high-sensitivity analysis of individual extracellular vesicles and particles. I will outline the workings of these two new flow cytometry platforms, describe their performance, and discuss the clinical questions we are addressing with these next-generation technologies.