Abstract

Effect of Plasma Exosome Isolation Methods on Multi-Omic Profiles in Cancer Patients and Healthy Controls

Liang Wang, Senior Member of Tumor Biology, Moffitt Cancer Center

Circulating exosomes in the blood are promising tools for biomarker discovery in cancer. Due to their heterogeneity, different isolation methods may enrich distinct exosome cargos generating different omic profiles. In this presentation, I will share our recent study on the effects of plasma exosome isolation methods on multi-omic profiles in cancer patients and healthy controls. This study evaluated three exosome isolation methods including size exclusion chromatography, lectin binding, and TIM4 binding. For each exosome isolation method, molecular profiling analysis was performed included lipidome, metabolome, proteome and transcriptome. Our study shows clear difference in molecular profiles of the exosomes isolated using different methods. Our findings suggest that specific isolation methods select subpopulations which may significantly impact cancer biomarker discovery.