Abstract

Mass-Density, Weight and Size as Novel Biophysical Parameters to Analyze and Sort 3D Cell Culture

Andrea Cristaldi, Senior R&D Specialist, CellDynamics isrl

Introducing a unique approach for the biophysical characterization of 3D cell cultures, dedicated to Live and/or fixed 3D samples ranging from 50 µm sized cell clusters to larger spheroids or organoids up to 500 µm in diameter. The Flow-Based Gravimetric method allows measuring MASS DENSITY, WEIGHT and SIZE of 3D CELL CULTURE as well as performing BIOPHYSICAL-BASED SORTING in a LABEL-FREE manner, preserving samples' STERILITY and VIABILITY. An overview of the growing verified applications will demonstrate how it is now possible to start correlating biological events with biophysical properties, helping researchers gather crucial compactness- and cross-sectional-information, as well as support and improve their workflows. This includes quantitatively monitoring samples’ growth or maturation, standardization and QC prior to further downstream analysis, cell infiltration rates prediction, extracellular matrix production, co-culture growth monitoring, viability trends, drug activity effects and so on. Most importantly, the custom mass-density sorting of a target sub-population, with preserved samples’ sterility and viability, would become the key factor to improve standardization for researchers that want to proceed further with specific downstream analysis minimizing workflow-depending biases.