Abstract

Extracellular Vesicle Detection and Calibration on the Cytek® Amnis® CellStream® and Cytek® Amnis® ImageStream® Systems

Robert Thacker, Imaging Sales and Applications Specialist, Cytek Biosciences

The quantification and characterization of extracellular vesicles is challenging due to their size. High magnification microscopy can analyze EVs, but has very low throughput. Flow cytometry is currently the only method building in rigor and reproducibility for single EV measurements, but older PMT-based flow cytometers have limitations in detecting EVS due to low sensitivity, cutting out the smallest of particles. Flow cytometry systems that use a charge coupled device (CCD)-camera have greatly enhanced sensitivity and increase the limit of detection. Today’s presentation will focus on two such CCD based systems – the Cytek® Amnis® CellStream® and Cytek® Amnis® ImageStream®  - and share results  from studies demonstrating the low limit of detection and the importance of calibration for more accurate sizing standardization of protocols within the field.