Abstract

Organoids with Reversed Biopolarity (ORBs): SARS-CoV-2 Drug Testing and Breast Cancer Progression

Shuichi Takayama, Professor, Georgia Research Alliance Eminent Scholar, and Price Gilbert, Jr. Chair in Regenerative Engineering and Medicine, Georgia Institute of Technology & Emory University School of Medicine

This presentation will describe production of sub-millimeter diameter, consistent size and shape, lung, kidney, and mammary organoids that have an inverted, apical-out geometry. These Organoids with Reversed Biopolarity (ORBs) are used in 384 well plates in a single-organoid-per-well format to test drugs and disease physiology. The airway ORBs are infected with high yields with multiple SARS-CoV-2 strains with Omicron variant showing highest viral replication and Delta giving the most inflammatory response. The ORBs also predict anti-viral drug efficacy correctly where conventional 2D cultures give false signals. Early-stage breast cancer progression model studies will also be discussed.