Abstract

Precision Medicine with Drug Susceptibility Test on Digital Microfluidics with 2D or 3D Organoid

Yanwei Jia, Associate Professor, University of Macau

Digital microfluidics (DMF) is a cutting edge technology for manipulating individual droplets on-chip based on the phenomenon of electro-wetting on dielectric. The utilization of electric signal for droplets control in DMF eliminates the bulky external pumps and valves in traditional channel-based microfluidics, making DMF an ideal system for point-of-care disease diagnosis. In this talk, I will introduce our recent progress in the development of digital microfluidics and its application in precision medicine, including single cell drug screening, wide-ranged flexible sample delivery on-chip controlled with external electric signal, and single or combinational multiple drug screening on-chip etc. We also built a portable DMF device for primary tumor cell drug susceptibility test. The in-vitro drug susceptibility results for instructing precision medicine has been validated in breast cancers in xenograft mice and clinical liver cancers with in-vivo tumor suppression treated by effective drugs on primary tumor cells screened on-chip. We have further generated 3D organoids on-chip for precision treatment. All these investigations have paved the way for automatic digital microfluidic system for precision medicine in the future.