Abstract

All Polymer Injection Molded Nanofluidic Devices with Nanoslits for High Quality at Low Cost Barcoding of Long Genome DNA

Rafael Taboryski, Professor, Technical University of Denmark

We present a fast and cheap methodology for imaging and barcoding DNA, stretched out in injection molded, polymer nanofluidic chips with channel dimensions of 110nm x 20µm. DNA has previously been optically mapped by barcoding using glass chips containing nanochannels [1,2] and –slits[3], and even in injection molded polymer chips containing nanochannels[4]. The glass chips are extremely expensive, making it necessary to use the same chip several time, causing possible cross contamination, while the polymer chips[4] have so far only been used with nanochannels, being inappropriate for sequencing of long (Mbp-size) genomes. In this work, we present an injection molded, all polymer device, fabricated in TOPAS COC-5013L-10 [5] containing an ultra-low aspect ratio nanoslit with height-to-width ratio of almost 1/200. These chips have a production time per chip and a unit price so low that they can be considered truly disposable. This removes all risks of cross contamination within experiments, as the polymer chips are only used once. These nanoslits, have been used for stretching out DNA-molecules of length of ~1.2Mbp, proving the first step required for optical mapping to see structural variations in the genome caused by mutations, such as insertions, deletions, and translations.