Abstract

Monitoring Dynamic Interactions of Tumor Cells with Tissue and Immune Cells in a Lab-on-a-Chip

Peter Ertl, Professor of Lab-on-a-Chip Systems, Vienna University of Technology

A complementary cell analysis method has been developed to assess the dynamic interactions of tumor cells with resident tissue and immune cells using optical light scattering and impedance spectroscopy to shed light on tumor cell behavior. The combination of electroanalytical and optical biosensing technologies integrated in a lab-on-a-chip allows for continuous, label-free and non-invasive probing of dynamic cell-to-cell interactions between adherent and non-adherent co-cultures, thus providing real-time insights into tumor cell responses under physiologically relevant conditions. While the study of adherent co-cultures is important for the understanding and suppression of tumor invasion, the analysis of tumor cell interactions with non-adherent immune cells plays a vital role in cancer immunotherapy research. For the first time the direct cell-to-cell interactions of tumor cells with bead-activated primary T cells were continuously assessed using an effector cell to target cell ratio of 10:1.