Abstract

High Content Screening of Organoid Cultures to Visualize and Quantify Intestinal Toxicity

Bram Herpers, COO, OcellO B V

Establishing organoids from human material enables development of 3D cell culture models for the intestinal epithelium to investigate physiological and toxicological mechanisms. At OcellO we combine a high throughput 3D human intestinal organoid culture platform with high content phenotype-based analysis to visualize and quantify the effects of compounds and treatment conditions on the epithelial integrity, injury and inflammation in a diverse (healthy and diseased) patient-derived organoid population.