Abstract

Characterizing 3D Cell Culture Models in Their Entirety Using High Content Confocal Imaging

Erin Edwards, Head of Services, Visikol

In this presentation, Visikol Head of Services Dr. Erin Edwards will discuss the challenges and limitations with characterizing 3D cell culture models from labeling and tissue processing to imaging and image analysis. The presentation will describe practical approaches to 3D cell culture characterization and how advanced imaging modalities such as high content confocal can be used to answer complex research questions. Specific applications in the fields of NASH, immuno-oncology and large molecule therapeutics will be discussed wherein spatial data can be combined with multiplex labeling to quantify T-cell migration, volumetric collagen deposition or therapeutic penetration.