Nanoplasmonic Platforms For Biosensing Applications
Amy Shen, Provost, Professor, Okinawa Institute of Science and Technology
Fabricating large-scale bioplasmonic materials at high-throughput is
important for the development of bio/chemical sensors and high
resolution nanomaterial based bioimaging tools. However, techniques
specific to large-scale synthesis of biocompatible nanoplasmonic
materials have found limited acceptance in industry due to their
time-consuming and complex fabrication procedures. Here, by exploiting
properties of reactive ions in a SF6 plasma environment, we assemble
nanoplasmonic substrates containing mushroom-like structures with SiO2
(insulator) stems and metal caps of gold (45-60 nm in total height, ~20
nm in width), evenly distributed with ~10 nm spacing on a glass
substrate. We demonstrate that our developed gold nanomushroom (Au NM)
substrate is biocompatible and sensitive for localized surface plasmon
resonance (LSPR) based biosensing applications. This nanoplasmonic
platform (coupled with microfluidics) is used for monitoring mitosis of
fibroblasts for 7 days, E. coli biofilm formation, protein/DNA based
immunoassays , and DNA polymerase activity in real-time.
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