Abstract

High-Throughput Brain Organoids Reveal Human Neurogenesis-Relevant Transcriptional Profiles

Milos Kostic, Scholar, Novartis Institutes for BioMedical Research

Recent breakthroughs in 3D brain organoid systems show that they can recapitulate aspects of human neurogenesis, such as cytoarchitecture, cell type heterogeneity, lineage progression and gene expression that are absent in 2D models. These properties mark them as attractive model system to study neurodevelopmental disorders. However, brain organoid research suffers from the lack of rigorous quality control for heterogeneity. To address this, we developed a high-throughput platform to generate organoids from multiple stem cell lines. We will present comprehensive quality control of brain organoids using single cell RNA sequencing (scRNA-seq) coupled with immunohistochemistry analysis.